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Image Search Results
Journal: mBio
Article Title: Evidence for the Role of a Second Fc-Binding Receptor in Placental IgG Transfer in Nonhuman Primates
doi: 10.1128/mbio.00341-23
Figure Lengend Snippet: Poor placental IgG MAb transfer is associated with a reduced binding to primate FcγR. (A) Multiplexed SPR sensorgrams depicting association and dissociation of major low-affinity rhesus macaque FcγR allotypic variants (rows) across mammalian (m) and plant (p) antibodies (columns); (B) sensorgrams showing binding of the major high-affinity rhesus macaque FcγR1 variant to mammal- and plant-produced forms of PGT121 defined by BLI; (C) equilibrium binding affinities of antibodies across low-affinity FcγR. K D values that could not be confidently estimated were set as exceeding 20 μM, the highest concentration assessed. (D) Plant- and mammal-derived PGT121 and PGT121-LS were assessed for the ability to induce phagocytosis in human THP-1 cells. (E) Levels of IL-2 (optical density at 450 nm [OD 450 ]) detected in supernatant of rhesus FcγR-expressing reporter cell lines induced by culture on antibody-coated plates.
Article Snippet: At the time that cells were added, 384-well high-binding plates (Corning 3700) were coated with 3 μg/mL
Techniques: Binding Assay, Variant Assay, Produced, Concentration Assay, Derivative Assay, Expressing